Composite

Part:BBa_K3308000:Design

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-09-30)


gpD-GIGGG-[IMPDH-1 N (1-55)]-[Npu-PCC73102 DnaE N]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 650
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemcial requrements for interactione between either ends of intein.


Source

The source of this composite part was sequences of Basic parts found through Genbank.

References